High-temperature-resistant lipase gene tllgold and application thereof

The invention discloses a high-temperature-resistant lipase gene tllgold and application thereof. The lipase gene tllgold is used for encoding high-temperature-resistant lipase, and the nucleotide sequence of the lipase gene tllgold is shown as SEQ ID NO: 1. According to the high-temperature-resistant lipase gene tllgold, on the basis of lipase TLL of original thermomyces lanuginosus, site-specific mutagenesis modification is performed on the TLL through artificial rational setting to obtain a modified lipase TLLGold amino acid sequence, then a nucleotide sequence of the lipase gene is artificially redesigned according to the amino acid sequence of the lipase TLLGold, a novel lipase gene tllgold sequence is designed, lipase TLLGold obtained through encoding of the lipase gene tllgold has the characteristic of high temperature resistance, 79% of enzyme activity can still be kept after the lipase TLLGold is placed in an environment of 80 DEG C for 12 h, and the lipase TLLGold can be suitable for various industr