Lactobacillus mixed high-density fermentation and preparation of lactobacillus compound bacterium preparation

The invention relates to lactobacillus mixed high-density fermentation and preparation of a lactobacillus compound bacterium preparation. Specifically, the invention provides a microbial fermentationmethod. The microbial fermentation method comprises the following steps: (1) respectively inoculating...

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Bibliographische Detailangaben
Hauptverfasser: PAN JIAMING, WANG JIE, LIU WEN, FENG JUNYIN, ZHANG QINGLIN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention relates to lactobacillus mixed high-density fermentation and preparation of a lactobacillus compound bacterium preparation. Specifically, the invention provides a microbial fermentationmethod. The microbial fermentation method comprises the following steps: (1) respectively inoculating an improved MRS liquid culture medium with one or more different seed bacteria, and performing culturing to obtain one or more different seed bacterial solutions; and (2) adding the one or more different seed bacterial solutions into a fermentation culture medium, and performing fermenting to obtain a fermentation solution. The improved MRS liquid culture medium comprises 12-18g/L of peptone, 12-18g/L of beef extracts, 7-15g/L of yeast extracts, 20-60g/L of glucose and water. According to themicrobial fermentation method disclosed by the invention, mixed fermentation of different lactobacillus can be performed at the same time, and in the mixed fermentation process, the different lactobacillus do not have a mutual