2, 4, 5-trichlorophenoxyacetic acid hapten, artificial antigen and application of 2, 4, 5-trichlorophenoxyacetic acid hapten and artificial antigen in immunodetection

The invention discloses a 2, 4, 5-trichlorophenoxyacetic acid hapten, an artificial antigen and an application of the 2, 4, 5-trichlorophenoxyacetic acid hapten in immunodetection. The arm introducedby the 2, 4, 5-trichlorophenoxyacetic acid hapten (2-(2-amino-3, 4, 6-trichlorophenoxy) acetic acid)...

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Hauptverfasser: SHI SONG, JIANG LINFENG, LI BIN, SU ZHENXIAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a 2, 4, 5-trichlorophenoxyacetic acid hapten, an artificial antigen and an application of the 2, 4, 5-trichlorophenoxyacetic acid hapten in immunodetection. The arm introducedby the 2, 4, 5-trichlorophenoxyacetic acid hapten (2-(2-amino-3, 4, 6-trichlorophenoxy) acetic acid) designed by the invention not only has an active group, but also can completely reserve a carboxylgroup of a target object to be detected, so that the electron cloud densities of the hapten and the target object to be detected are kept consistent, and the coupling is simple. The 2, 4, 5-trichlorophenoxyacetic acid artificial antigen and monoclonal antibody constructed on the basis are used for ELISA detection, the specificity is high, and an IC50 value is 4.9 [mu] g/L; when used in a fluorescence quantitative immunochromatography technology, the product has sensitivity of 3 [mu] g/L and excellent stability. 本发明公开了一种2,4,5-三氯苯氧乙酸半抗原、人工抗原及其在免疫检测中的应用。本发明设计的2,4,5-三氯苯氧乙酸半抗原(2-(2-胺基-3,4,6-三氯苯氧基)乙酸)引入的手臂不仅具有活性基团,还可以完整保留待测目