Bovine pasteurella multocida inactivated vaccine and preparation method thereof

The invention discloses a bovine pasteurella multocida inactivated vaccine and a preparation method thereof, and belongs to the technical field of veterinary biological products. The vaccine comprisesantigens and adjuvants, wherein the antigens are a bovine pasteurella multocida capsule type B C45-2 strain and a C47-2 strain. The preparation method comprises the following steps: performing inoculated culture on strains, performing shake culture, streaking a culture on an agar plate for culture, selecting bacterial colonies, inoculating a fresh blood slant with the selected bacterial colonies,performing culturing to obtain primary seeds, inoculating a culture medium with the primary seeds, and performing shake culture to obtain secondary seeds; mixing the secondary seeds, and performing inoculated culture to obtain zymophyte liquid, wherein the culture process is optimized, so that the antigen quantity is multiplied; and performing centrifuging to remove endotoxin, transferring bacterial sludge into normal sal