Streptomycete cello-oligosaccharide transport protein gene

The invention discloses a streptomycete cello-oligosaccharide transport protein gene, and belongs to the technical field of biology. The engineering strain for efficiently expressing the streptomycescello-oligosaccharide transport protein gene is successfully constructed by connecting the streptomyc...

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Bibliographische Detailangaben
Hauptverfasser: ZHOU BINGQING, LIU YI, BI DEWU, LIN LIHUA, PANG HAO, GUO YUAN, TANG HONGCHI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a streptomycete cello-oligosaccharide transport protein gene, and belongs to the technical field of biology. The engineering strain for efficiently expressing the streptomycescello-oligosaccharide transport protein gene is successfully constructed by connecting the streptomyces cello-oligosaccharide transport protein gene to a pET-28a vector, transferring the streptomycescello-oligosaccharide transport protein gene into a competent target strain and carrying out induced expression culture, and thermodynamic experiments prove that the engineering strain can be efficiently combined with cello-oligosaccharide, especially cellobiose, so that a tool is provided for further industrial utilization of cellulose. 本发明公开了一种链霉菌纤维寡糖转运蛋白基因,属于生物技术领域。本发明通过将链霉菌纤维寡糖转运蛋白基因连接到pET-28a载体上后转入感受态目的菌株并进行诱导表达培养,成功构建了高效表达链霉菌纤维寡糖转运蛋白的工程菌株,并通过热力学实验证明了其能够高效结合纤维寡糖,尤其是纤维二糖,为产业上进一步利用纤维素提供了工具。