Engineering bacterium for producing hydroxytyrosol

The invention discloses an engineering bacterium for producing hydroxytyrosol. The engineering bacterium simultaneously expresses hydroxylase for catalyzing tyrosol to generate hydroxytyrosol and coenzyme for reducing NAD to generate NADH, and an NAD coenzyme cyclic regeneration system is constructed. The engineering bacterium is used for producing hydroxytyrosol by taking tyrosol as a substrate, the process and the extraction method are simple, the product is single, the yield and the yield are obviously improved, and the engineering bacterium has good industrial application prospects. 本发明公开了一种用于生产羟基酪醇的工程菌，该工程菌同时表达将酪醇催化生成羟基酪醇的羟化酶以及将NAD+还原生成NADH的辅酶，构建了NAD辅酶循环再生体系。利用该工程菌以酪醇为底物生产得到羟基酪醇，过程及提取方法简单且产品单一，产量和产率都得到明显的提高，具有良好的工业化应用前景。