Sample detection device and manufacturing method thereof

A sample detection device with a flow channel comprises an optical signal detection device for receiving and detecting an incident optical signal, wherein the optical signal detection device comprisesa plurality of image sensor chip units manufactured in the same substrate; and a flow channel device arranged on the upper surface of the optical signal detection device and comprising a shell, wherein at least one sample flow inlet and at least one sample flow outlet are formed in the shell, and at least one flow channel is formed between the lower surface of the shell and the upper surface of the optical signal detection device, each flow channel corresponding to at least one sample flow inlet and at least one sample flow outlet, and corresponding to at least one image sensor chip unit. Theflow channels and the corresponding sample flow inlets and sample flow outlets are arranged in a manner that biological samples can flow into the corresponding flow channels through the sample flow inlets, reach the surfaces