Primer group, kit and method for detecting SARS-CoV-2 novel coronavirus N gene

The invention discloses a primer group, kit and method for detecting a SARS-CoV-2 novel coronavirus N gene. The primer group for detecting the SARS-CoV-2 novel coronavirus N gene comprises an outer primer F3-N1, an outer primer B3-N1, an inner primer FIP-N1, an inner primer BIP-N1, a loop primer LF-N1 and a loop primer LB-N1. The primer group for detecting the SARS-CoV-2 novel coronavirus N gene can specifically amplify SARS-CoV-2 novel coronavirus N gene segments; 20 copies can be detected minimally; and compared with a conventional PCR method, the primer group has the advantage that the detection sensitivity is greatly improved, so that whether SARS-CoV-2 novel coronavirus exists in a detected sample or not is judged. 本发明公开了一种用于检测SARS-CoV-2新型冠状病毒N基因的引物组、试剂盒及方法。一种用于检测SARS-CoV-2新型冠状病毒N基因的引物组，包括：外引物F3-N1，外引物B3-N1，内引物FIP-N1，内引物BIP-N1，环引物LF-N1，环引物LB-N1。本发明用于检测SARS-CoV-2新型冠状病毒N基因的引物组可特异性扩增SARS-CoV-2新型冠状病毒N基因片段，最低均可检测到20个拷贝数，与常规PCR方法相比大大提高了检测灵敏度，从而判断检测样品中是否存在SARS-CoV-2新型冠状病毒。