Recombinant lysozyme LYZ-2 with improved enzymatic activity as well as mutant and application thereof
The invention provides a recombinant lysozyme LYZ-2 with improved enzymatic activity as well as a mutant and application thereof. According to the invention, a gene sequence of natural lysozyme is optimized, random mutation is carried out by utilizing an error-prone PCR method on the basis, recombin...
Gespeichert in:
| Hauptverfasser: | , , , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | XIAO ZHIZHUANG FU WUBING YANG YUCHAO XUE HAIZHAO YU WENJUN LYU WEI FANG ANRAN ZHANG DAWEI |
| description | The invention provides a recombinant lysozyme LYZ-2 with improved enzymatic activity as well as a mutant and application thereof. According to the invention, a gene sequence of natural lysozyme is optimized, random mutation is carried out by utilizing an error-prone PCR method on the basis, recombinant lysozyme LYZ-2 and lysozyme mutant LYZ-2-m are obtained, and the enzyme activity of the lysozymeLYZ-2 and lysozyme mutant LYZ-2-m is obviously improved. The obtained recombinant lysozyme LYZ-2 and lysozyme mutant LYZ-2-m can be added into feed for breeding animals, the growth condition of animals is improved, and the survival rate is increased. According to the invention, theoretical and practical foundations are laid for large-scale production of lysozyme through a genetic engineering method, and the lysozyme obtained is good in property and has good application prospects in the breeding industry.
本发明提供了一种酶活性提高的重组溶菌酶LYZ-2及其突变体和应用。本本发明优化了天然溶菌酶的基因序列，并在此基础上利用易错PCR方法进行了随机突变，获得了重组溶菌酶LYZ-2及溶菌酶突变体LYZ-2-m，其酶活均得到了显著的提高 |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN112111474A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN112111474A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN112111474A3</originalsourceid><addsrcrecordid>eNqNjDsKwkAQQNNYiHqH8QApNgasJSgWYiE22oRxMyED-yM7JqynNwEPYPWK93jLjG6kvX2xQydgUvSfZAkuj2dewMjSAdvQ-4EaIDcpFNaAWnhgSYARRjJmpn3LfEDXAIZgWE-ldyAd9eTbdbZo0UTa_LjKtqfjvTrnFHxNMaAmR1JXV6UKpVS5Lw-7f5ovNDI_QA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Recombinant lysozyme LYZ-2 with improved enzymatic activity as well as mutant and application thereof</title><source>esp@cenet</source><creator>XIAO ZHIZHUANG ; FU WUBING ; YANG YUCHAO ; XUE HAIZHAO ; YU WENJUN ; LYU WEI ; FANG ANRAN ; ZHANG DAWEI</creator><creatorcontrib>XIAO ZHIZHUANG ; FU WUBING ; YANG YUCHAO ; XUE HAIZHAO ; YU WENJUN ; LYU WEI ; FANG ANRAN ; ZHANG DAWEI</creatorcontrib><description>The invention provides a recombinant lysozyme LYZ-2 with improved enzymatic activity as well as a mutant and application thereof. According to the invention, a gene sequence of natural lysozyme is optimized, random mutation is carried out by utilizing an error-prone PCR method on the basis, recombinant lysozyme LYZ-2 and lysozyme mutant LYZ-2-m are obtained, and the enzyme activity of the lysozymeLYZ-2 and lysozyme mutant LYZ-2-m is obviously improved. The obtained recombinant lysozyme LYZ-2 and lysozyme mutant LYZ-2-m can be added into feed for breeding animals, the growth condition of animals is improved, and the survival rate is increased. According to the invention, theoretical and practical foundations are laid for large-scale production of lysozyme through a genetic engineering method, and the lysozyme obtained is good in property and has good application prospects in the breeding industry.
本发明提供了一种酶活性提高的重组溶菌酶LYZ-2及其突变体和应用。本本发明优化了天然溶菌酶的基因序列，并在此基础上利用易错PCR方法进行了随机突变，获得了重组溶菌酶LYZ-2及溶菌酶突变体LYZ-2-m，其酶活均得到了显著的提高</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FODDER ; FOODS OR FOODSTUFFS ; HUMAN NECESSITIES ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20201222&DB=EPODOC&CC=CN&NR=112111474A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,781,886,25566,76549</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20201222&DB=EPODOC&CC=CN&NR=112111474A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>XIAO ZHIZHUANG</creatorcontrib><creatorcontrib>FU WUBING</creatorcontrib><creatorcontrib>YANG YUCHAO</creatorcontrib><creatorcontrib>XUE HAIZHAO</creatorcontrib><creatorcontrib>YU WENJUN</creatorcontrib><creatorcontrib>LYU WEI</creatorcontrib><creatorcontrib>FANG ANRAN</creatorcontrib><creatorcontrib>ZHANG DAWEI</creatorcontrib><title>Recombinant lysozyme LYZ-2 with improved enzymatic activity as well as mutant and application thereof</title><description>The invention provides a recombinant lysozyme LYZ-2 with improved enzymatic activity as well as a mutant and application thereof. According to the invention, a gene sequence of natural lysozyme is optimized, random mutation is carried out by utilizing an error-prone PCR method on the basis, recombinant lysozyme LYZ-2 and lysozyme mutant LYZ-2-m are obtained, and the enzyme activity of the lysozymeLYZ-2 and lysozyme mutant LYZ-2-m is obviously improved. The obtained recombinant lysozyme LYZ-2 and lysozyme mutant LYZ-2-m can be added into feed for breeding animals, the growth condition of animals is improved, and the survival rate is increased. According to the invention, theoretical and practical foundations are laid for large-scale production of lysozyme through a genetic engineering method, and the lysozyme obtained is good in property and has good application prospects in the breeding industry.
本发明提供了一种酶活性提高的重组溶菌酶LYZ-2及其突变体和应用。本本发明优化了天然溶菌酶的基因序列，并在此基础上利用易错PCR方法进行了随机突变，获得了重组溶菌酶LYZ-2及溶菌酶突变体LYZ-2-m，其酶活均得到了显著的提高</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FODDER</subject><subject>FOODS OR FOODSTUFFS</subject><subject>HUMAN NECESSITIES</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>THEIR TREATMENT, NOT COVERED BY OTHER CLASSES</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2020</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjDsKwkAQQNNYiHqH8QApNgasJSgWYiE22oRxMyED-yM7JqynNwEPYPWK93jLjG6kvX2xQydgUvSfZAkuj2dewMjSAdvQ-4EaIDcpFNaAWnhgSYARRjJmpn3LfEDXAIZgWE-ldyAd9eTbdbZo0UTa_LjKtqfjvTrnFHxNMaAmR1JXV6UKpVS5Lw-7f5ovNDI_QA</recordid><startdate>20201222</startdate><enddate>20201222</enddate><creator>XIAO ZHIZHUANG</creator><creator>FU WUBING</creator><creator>YANG YUCHAO</creator><creator>XUE HAIZHAO</creator><creator>YU WENJUN</creator><creator>LYU WEI</creator><creator>FANG ANRAN</creator><creator>ZHANG DAWEI</creator><scope>EVB</scope></search><sort><creationdate>20201222</creationdate><title>Recombinant lysozyme LYZ-2 with improved enzymatic activity as well as mutant and application thereof</title><author>XIAO ZHIZHUANG ; FU WUBING ; YANG YUCHAO ; XUE HAIZHAO ; YU WENJUN ; LYU WEI ; FANG ANRAN ; ZHANG DAWEI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN112111474A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2020</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FODDER</topic><topic>FOODS OR FOODSTUFFS</topic><topic>HUMAN NECESSITIES</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>THEIR TREATMENT, NOT COVERED BY OTHER CLASSES</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>XIAO ZHIZHUANG</creatorcontrib><creatorcontrib>FU WUBING</creatorcontrib><creatorcontrib>YANG YUCHAO</creatorcontrib><creatorcontrib>XUE HAIZHAO</creatorcontrib><creatorcontrib>YU WENJUN</creatorcontrib><creatorcontrib>LYU WEI</creatorcontrib><creatorcontrib>FANG ANRAN</creatorcontrib><creatorcontrib>ZHANG DAWEI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>XIAO ZHIZHUANG</au><au>FU WUBING</au><au>YANG YUCHAO</au><au>XUE HAIZHAO</au><au>YU WENJUN</au><au>LYU WEI</au><au>FANG ANRAN</au><au>ZHANG DAWEI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Recombinant lysozyme LYZ-2 with improved enzymatic activity as well as mutant and application thereof</title><date>2020-12-22</date><risdate>2020</risdate><abstract>The invention provides a recombinant lysozyme LYZ-2 with improved enzymatic activity as well as a mutant and application thereof. According to the invention, a gene sequence of natural lysozyme is optimized, random mutation is carried out by utilizing an error-prone PCR method on the basis, recombinant lysozyme LYZ-2 and lysozyme mutant LYZ-2-m are obtained, and the enzyme activity of the lysozymeLYZ-2 and lysozyme mutant LYZ-2-m is obviously improved. The obtained recombinant lysozyme LYZ-2 and lysozyme mutant LYZ-2-m can be added into feed for breeding animals, the growth condition of animals is improved, and the survival rate is increased. According to the invention, theoretical and practical foundations are laid for large-scale production of lysozyme through a genetic engineering method, and the lysozyme obtained is good in property and has good application prospects in the breeding industry.
本发明提供了一种酶活性提高的重组溶菌酶LYZ-2及其突变体和应用。本本发明优化了天然溶菌酶的基因序列，并在此基础上利用易错PCR方法进行了随机突变，获得了重组溶菌酶LYZ-2及溶菌酶突变体LYZ-2-m，其酶活均得到了显著的提高</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN112111474A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FODDER FOODS OR FOODSTUFFS HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE |
| title | Recombinant lysozyme LYZ-2 with improved enzymatic activity as well as mutant and application thereof |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-17T16%3A15%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=XIAO%20ZHIZHUANG&rft.date=2020-12-22&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN112111474A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |