Recombinant lysozyme LYZ-2 with improved enzymatic activity as well as mutant and application thereof

The invention provides a recombinant lysozyme LYZ-2 with improved enzymatic activity as well as a mutant and application thereof. According to the invention, a gene sequence of natural lysozyme is optimized, random mutation is carried out by utilizing an error-prone PCR method on the basis, recombinant lysozyme LYZ-2 and lysozyme mutant LYZ-2-m are obtained, and the enzyme activity of the lysozymeLYZ-2 and lysozyme mutant LYZ-2-m is obviously improved. The obtained recombinant lysozyme LYZ-2 and lysozyme mutant LYZ-2-m can be added into feed for breeding animals, the growth condition of animals is improved, and the survival rate is increased. According to the invention, theoretical and practical foundations are laid for large-scale production of lysozyme through a genetic engineering method, and the lysozyme obtained is good in property and has good application prospects in the breeding industry. 本发明提供了一种酶活性提高的重组溶菌酶LYZ-2及其突变体和应用。本本发明优化了天然溶菌酶的基因序列，并在此基础上利用易错PCR方法进行了随机突变，获得了重组溶菌酶LYZ-2及溶菌酶突变体LYZ-2-m，其酶活均得到了显著的提高