Research method of protection mechanism of BMSC-Exo on high glucose induced HK2 cell damage

The invention belongs to the technical field of diabetic nephropathy treatment, and particularly relates to a research method of a protection mechanism of BMSC-Exo on high glucose induced HK2 cell damage. The experimental steps of the protection mechanism of BMSC-Exo on high glucose induced HK2 cell damage include HK2 cell grouping and processing, construction of a miR-886-5p knocked-out HK2 cellline by a Crispr/Cas9 technology, an RNA pull down technology, an FISH experiment, DN establishment and PAS glycogen staining. Bioinformatics prediction is combined with a luciferase reporter vector experiment to prove the interaction between miR-886-5p and a target gene, the RNA pull down experiment and the FISH experiment further prove the binding relationship between miR-886-5 p and the targetgene, and a cure experiment proves that the target gene can reverse the function of miR-886-5p and confirm the mechanism of miR-886-5p in HK2 cells. 本发明公开的属于糖尿病肾病治疗技术领域，具体为BMSC-Exo对高糖诱导HK2细胞损伤保护机制的研究方法，该BMSC-Exo对高糖诱导HK2细胞损伤的保护