Detection method of gelatin medicinal material

The invention discloses a detection method of gelatin medicinal materials. According to the detection method, trypsin is adopted for enzyme digestion of the gelatin medicinal material, so that specific polypeptide is released from collagen; then a liquid chromatography triple quadrupole mass spectrometer is adopted for detection, and if it is detected that the sample is corresponded to the specific polypeptide retentiontime, the parent ions and the daughter ions, determining that the species source of the gelatin medicinal material sample is consistent with the species corresponding to the specific polypeptide; and determining the adulteration proportion of the doped sample according to the peak area ratio of the specific polypeptide. The method has the advantages of strong specificity, high sensitivity, simple operation and the like, and can be used for identification and quality control of the gelatin medicinal materials. 本发明公开了一种胶类药材的检测方法，本发明采用胰蛋白酶酶切胶类药材，使专属性多肽从胶原蛋白中释放，而后采用液相色谱-三重四极杆质谱仪检测，如果检测出与专属性多肽保留时间、母