Polygene methylation detection kit for nasopharyngeal carcinoma diagnosis
The invention belongs to the field of molecular diagnosis, and particularly relates to a polygene methylation detection kit for the nasopharyngeal carcinoma diagnosis. The kit comprises a p16 gene extraction reagent group, a p16 gene methylation modification reagent group, a fluorescent quantitative...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention belongs to the field of molecular diagnosis, and particularly relates to a polygene methylation detection kit for the nasopharyngeal carcinoma diagnosis. The kit comprises a p16 gene extraction reagent group, a p16 gene methylation modification reagent group, a fluorescent quantitative PCR detection reagent group and a p16 gene methylation level calibrator. Compared with the prior art, the kit has the beneficial effects that the p16 gene in peripheral blood of a patient is extracted through superparamagnetic beads, the detection is carried out through fluorescent quantitative PCR, a detection sample is easier to obtain, the DNA extraction is simpler, more convenient and faster, and the commercialized application and popularization of the kit are facilitated; in addition, methylation detection primers adopted by the kit can more efficiently detect the methylation level of the p16 gene; and moreover, the p16 gene methylation level calibrator in the kit can ensure the reliability of a detection res |
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