Polygene methylation detection kit for nasopharyngeal carcinoma diagnosis

The invention belongs to the field of molecular diagnosis, and particularly relates to a polygene methylation detection kit for the nasopharyngeal carcinoma diagnosis. The kit comprises a p16 gene extraction reagent group, a p16 gene methylation modification reagent group, a fluorescent quantitative...

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Hauptverfasser: QIN HAIMEI, WEI GUIJIANG, WANG CHUNFANG, YANG FENGLIAN, WANG JUNLI, WEI YUXIA, PANG XIAOXIA, SONG YANLUN, CHEN XINGHONG, CHEN WENCHENG, SHI FENG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the field of molecular diagnosis, and particularly relates to a polygene methylation detection kit for the nasopharyngeal carcinoma diagnosis. The kit comprises a p16 gene extraction reagent group, a p16 gene methylation modification reagent group, a fluorescent quantitative PCR detection reagent group and a p16 gene methylation level calibrator. Compared with the prior art, the kit has the beneficial effects that the p16 gene in peripheral blood of a patient is extracted through superparamagnetic beads, the detection is carried out through fluorescent quantitative PCR, a detection sample is easier to obtain, the DNA extraction is simpler, more convenient and faster, and the commercialized application and popularization of the kit are facilitated; in addition, methylation detection primers adopted by the kit can more efficiently detect the methylation level of the p16 gene; and moreover, the p16 gene methylation level calibrator in the kit can ensure the reliability of a detection res