Preparation method of recombinant novel coronavirus subunit vaccine

The invention relates to the field of vaccine preparation, in particular to a preparation method of a recombinant novel coronavirus subunit vaccine. The method comprises the following steps: transfecting plasmids into which a nucleic acid fragment corresponding to RBD-TT recombinant protein is inser...

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Bibliographische Detailangaben
Hauptverfasser:	LYU HANGJUN, QIN CHUAN, GAO FU, XU WENBO, ZHUANG FANGCHENG, LU SHAOHONG, WU GUIZHEN, MAO ZI'AN, HOU YUNDE, BI SHENGLI, MAO JIANGSEN, CHEN GANG, SU QIUDONG, YI YAO, GAO MENG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the field of vaccine preparation, in particular to a preparation method of a recombinant novel coronavirus subunit vaccine. The method comprises the following steps: transfecting plasmids into which a nucleic acid fragment corresponding to RBD-TT recombinant protein is inserted into Escherichia coli; culturing the Escherichia coli to enable the Escherichia coli to expressthe RBD-TT fusion protein, and breaking thalli and separating an inclusion body crude extract of the RBD-TT recombinant protein; dissolving the inclusion body crude extract with a urea-containing denaturing solution, and then performing purifying through anion exchange chromatography to obtain an RBD-TT recombinant protein crude pure sample; diluting the RBD-TT recombinant protein crude pure sample with a diluent; performing filtering, and performing renaturation with a renaturation solution to obtain renaturation protein; and purifying the recombinant protein renaturation protein through anion exchange chromatography