Brucella recombinase mediated isothermal nucleic acid amplification kit

The invention discloses a brucella recombinase mediated isothermal nucleic acid amplification kit. The brucella recombinase mediated isothermal nucleic acid amplification kit comprises a forward primer BrucellaF, a reverse primer BrucellaR and a probe BrucellaP marked with THF residues. Based on the technical scheme, the amplification temperature of the isothermal nucleic acid amplification kit is39 DEG C, and the amplification time is 20 minutes; at least 100 copies of brucella samples can be detected; and the specificity is high. Compared with other PCR methods, the kit provided by the invention has the advantages that complex instruments and excellent laboratories are not needed; only one constant-temperature fluorescence amplification instrument is needed; the operation is simple andconvenient; meanwhile, the reaction time is short, and the amplification can be completed in only 15 to 20 minutes; and both the specificity and the sensitivity meet the requirements. The brucella recombinase mediated isotherm