Specific SSR labelled primer for purity identification of rape Pol-CMS hybrid seeds or parent seeds of rape Pol-CMS hybrid seeds and application of specific SSR labelled primer

The invention discloses a specific SSR labelled primer for purity identification of rape Pol-CMS hybrid seeds or parent seeds of the rape Pol-CMS hybrid seeds. The specific SSR labelled primer comprises a forward primer F and a reverse primer R, wherein the forward primer F is 5'-AGTTAGCTCTCTCT...

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Bibliographische Detailangaben
Hauptverfasser:	LI BAO, QU LIANG, LI MEI, WANG TONGHUA, GUO YIMING, FAN LIANYI, LIU XINHONG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a specific SSR labelled primer for purity identification of rape Pol-CMS hybrid seeds or parent seeds of the rape Pol-CMS hybrid seeds. The specific SSR labelled primer comprises a forward primer F and a reverse primer R, wherein the forward primer F is 5'-AGTTAGCTCTCTCTCTCTCTCTCTGTGTTTGC-3'; and the reverse primer R is 5'-CTACAGCTCGATTAGGGATCGT-3'. The specific SSR labelled primer has high universality and has a higher practical significance in purity identification of hybrid seeds or parent seeds of the hybrid seeds. The invention also discloses an application of thespecific SSR labelled primer in judgement of fertility of rape Pol-CMS seeds and purity identification of rape Pol-CMS hybrid seeds or parent seeds of the rape Pol-CMS hybrid seeds; the specific SSR labelled primer is simple in operation, accurate in identification result and high in universality, can greatly simplify purity identification of new varieties and new combinations, and provides new identification technology a