METHOD FOR DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELL LINES IN SUSPENSION CULTURE

Described herein is a method of differentiation of pluripotent stem cells into hematopoietic precursor cells, hematopoietic progenitor cells, erythroblast cells, and enucleated erythroblast cells, wherein the method is carried out under suspension agitation, and a GSK-3-inhibitor or a Wnt pathway activator is added during a stage of mesoderm induction. In the preferred embodiment, the pluripotentstem cells are expanded in agitated microcarrier culture and the GSK-3-inhibitior is CHIR99021. Also disclosed herein are cell culture media for use in the methods disclosed herein, as well as kits for performing the same. 本文描述了将多能干细胞分化为造血前体细胞、造血祖细胞、成红细胞和去核成红细胞的方法，其中所述方法在悬浮搅动下进行，并且在中胚层诱导阶段期间加入GSK-3-抑制剂或Wnt通路激活剂。在优选的实施方案中，多能干细胞在搅动的微载体培养物中扩增，且GSK-3抑制剂是CHIR99021。本文还公开了用于本文公开的方法的细胞培养基，以及用于进行所述方法的试剂盒。