Quantitative detection method, internal standard and kit for nucleic acid of androgen receptor splicing variant-7

The invention discloses a quantitative detection method, an internal standard and a kit for nucleic acid of an androgen receptor splicing variant-7. The kit comprises a primer probe combination related to AR-V7 quantitative detection, the internal standard and a reagent related to AR-V7 RT-PCR metho...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	ZHANG YANAN, QIAO CHUNXIAO, WANG XIAONAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention discloses a quantitative detection method, an internal standard and a kit for nucleic acid of an androgen receptor splicing variant-7. The kit comprises a primer probe combination related to AR-V7 quantitative detection, the internal standard and a reagent related to AR-V7 RT-PCR method detection. The kit can complete the quantitative detection of AR-V7 in a single tube, the quantitative result is accurate, the conventional quantitative operation process is greatly simplified, the efficiency is improved, and the risk of detection errors is also reduced. 本发明公开了雄激素受体剪接变异体-7核酸定量检测方法、内标及试剂盒。所述试剂盒包括AR-V7定量检测相关的引物探针组合、内标以及AR-V7 RT-PCR法检测相关试剂。且所述试剂盒可在单管内完成AR-V7的定量检测，定量结果准确，极大的简化了常规的定量操作流程，提高了效率，也降低了检测失误的风险。