Primer group, kit and detection method for detecting escherichia coli and salmonella in medicine

The invention relates to the technical field of specific detection of escherichia coli and salmonella in medicines, and provides a primer group, a kit and a detection method for detecting escherichiacoli and salmonella in medicines. The primer group comprises uidA, invA and 16SrDNA. By adopting the primer group, the beta-glucosidase gene of escherichia coli, the invasion protein A gene of salmonella and the gene of 16SrRNA subunit in a drug can be detected through multiple PCR; whether the medicine contains escherichia coli, salmonella and other bacteria or not is detected by detecting whether the medicine contains the beta-glucosidase gene, invasion protein A gene and 16SrRNA subunit gene or not. 本发明涉及特异性检测药物中大肠杆菌和沙门氏菌的技术领域，提供了用于药物中大肠杆菌、沙门氏菌检测的引物组、试剂盒和检测方法。该引物组包括uidA、invA和16SrDNA。采用该引物组通过多重PCR可以检测药物中的大肠杆菌的β-葡萄糖苷酶基因、沙门氏菌的侵袭蛋白A基因以及16SrRNA亚基的基因，通过检测药物中是否含有β-葡萄糖苷酶基因、侵袭蛋白A基因以及16SrRNA亚基的基因来检测药物中是否含有大肠杆菌和沙门氏菌等细菌。