Method for preparing chenodeoxycholic acid by extraction complexing process

The invention relates to a method for preparing chenodeoxycholic acid by an extraction complexing process. The method comprises the following steps: 1, extracting; 2, concentrating; 3, complexing; 4,removing magnesium; 5, crystallizing; 6, recrystallizing for obtaining the chenodeoxycholic acid finished product. According to the method, impurities such as sterols and proteins in chenodeoxycholicacid are extracted with petroleum ether, then ethyl ester is washed with weak alkaline water to remove hyocholic acid impurities in chenodeoxycholic acid, chenodeoxycholic acid is easy to complex under high-temperature catalysis of ethyl acetate, and complete separation of chenodeoxycholic acid is achieved. The method has the beneficial effects that the yield is high and is 20-25%, the melting point is high and is 160-170 DEG C, and the content of high-purity products is 98% or above through HPLC detection. The method has the characteristics of simplicity in operation, short process flow, reduction of organic reagent c