Fermentation method for producing high-activity recombinant human interferon alpha2b by utilizing microorganisms

The invention provides a fermentation method for producing high-activity recombinant human interferon alpha2b by utilizing microorganisms, and belongs to the technical field of fermentation engineering. According to the fermentation method, recombinant escherichia coli BL21 is taken as a fermentation strain; after undergoing primary seed culture and secondary seed culture, the recombinant escherichia coli is inoculated to an escherichia coli culture medium for fermentation culture; after OD1.0600 reaches 5.0-20.0, an inducer is added for cooling induction, and the concentration of a carbon source in fermentation broth is controlled by flow feeding, and culture is performed for 12-20h. The fermentation method concentrates on improving the culture medium, a culture process and induction conditions; based on an optimized fermentation formula and culture conditions, the culture effect is significantly improved; meanwhile, cooling induction is performed in the fermentation process, so thatcorrect folding of protei