Gene expression regulation and control method and system based on Type I-F CRISPR/ Cas
The invention discloses a gene expression regulation and control method and system based on Type I-F CRISPR/ Cas. The first study shows that a Cascade compound, such as a PaeCascade compound, belonging to a Type I-F CRISPR/ Cas system can be efficiently combined with a target site in a mammalian cel...
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| creator | LIANG PUPING SONG YANGZHOU LIU JIAQI CHEN YUXI |
| description | The invention discloses a gene expression regulation and control method and system based on Type I-F CRISPR/ Cas. The first study shows that a Cascade compound, such as a PaeCascade compound, belonging to a Type I-F CRISPR/ Cas system can be efficiently combined with a target site in a mammalian cell; and meanwhile, the Type I-F CRISPR/ Cas system is subjected to mammalian expression system optimization, so that a transcription activation factor or a transcription inhibition factor can be efficiently recruited to the target site in the mammalian cell, and a transcription activation system anda transcription inhibition system are successfully constructed. The application of the Type I-F CRISPR/ Cas system in the aspect of gene regulation and control of the mammalian cell becomes possible,and a necessary tool is provided for gene transcription regulation and control based on the Type I-F CRISPR/ Cas system.
本发明公开了一种基于Type I-F CRISPR/Cas的基因表达调控方法和基因表达调控系统。本发明首次研究显示隶属于Type I-F CRISPR/Cas系统的Cascade复合物，如PaeCascade复 |
| format | Patent |
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本发明公开了一种基于Type I-F CRISPR/Cas的基因表达调控方法和基因表达调控系统。本发明首次研究显示隶属于Type I-F CRISPR/Cas系统的Cascade复合物，如PaeCascade复</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2020</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20201124&DB=EPODOC&CC=CN&NR=111979240A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20201124&DB=EPODOC&CC=CN&NR=111979240A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIANG PUPING</creatorcontrib><creatorcontrib>SONG YANGZHOU</creatorcontrib><creatorcontrib>LIU JIAQI</creatorcontrib><creatorcontrib>CHEN YUXI</creatorcontrib><title>Gene expression regulation and control method and system based on Type I-F CRISPR/ Cas</title><description>The invention discloses a gene expression regulation and control method and system based on Type I-F CRISPR/ Cas. The first study shows that a Cascade compound, such as a PaeCascade compound, belonging to a Type I-F CRISPR/ Cas system can be efficiently combined with a target site in a mammalian cell; and meanwhile, the Type I-F CRISPR/ Cas system is subjected to mammalian expression system optimization, so that a transcription activation factor or a transcription inhibition factor can be efficiently recruited to the target site in the mammalian cell, and a transcription activation system anda transcription inhibition system are successfully constructed. The application of the Type I-F CRISPR/ Cas system in the aspect of gene regulation and control of the mammalian cell becomes possible,and a necessary tool is provided for gene transcription regulation and control based on the Type I-F CRISPR/ Cas system.
本发明公开了一种基于Type I-F CRISPR/Cas的基因表达调控方法和基因表达调控系统。本发明首次研究显示隶属于Type I-F CRISPR/Cas系统的Cascade复合物，如PaeCascade复</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2020</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZAhzT81LVUitKChKLS7OzM9TKEpNL81JLAExE_NSFJLz80qK8nMUclNLMvJTwELFlcUlqbkKSYnFqSkKQGUhlQWpCp66bgrOQZ7BAUH6Cs6JxTwMrGmJOcWpvFCam0HRzTXE2UM3tSA_PrW4IDEZaG1JvLOfoaGhpbmlkYmBozExagB9hjfK</recordid><startdate>20201124</startdate><enddate>20201124</enddate><creator>LIANG PUPING</creator><creator>SONG YANGZHOU</creator><creator>LIU JIAQI</creator><creator>CHEN YUXI</creator><scope>EVB</scope></search><sort><creationdate>20201124</creationdate><title>Gene expression regulation and control method and system based on Type I-F CRISPR/ Cas</title><author>LIANG PUPING ; SONG YANGZHOU ; LIU JIAQI ; CHEN YUXI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN111979240A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2020</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LIANG PUPING</creatorcontrib><creatorcontrib>SONG YANGZHOU</creatorcontrib><creatorcontrib>LIU JIAQI</creatorcontrib><creatorcontrib>CHEN YUXI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LIANG PUPING</au><au>SONG YANGZHOU</au><au>LIU JIAQI</au><au>CHEN YUXI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Gene expression regulation and control method and system based on Type I-F CRISPR/ Cas</title><date>2020-11-24</date><risdate>2020</risdate><abstract>The invention discloses a gene expression regulation and control method and system based on Type I-F CRISPR/ Cas. The first study shows that a Cascade compound, such as a PaeCascade compound, belonging to a Type I-F CRISPR/ Cas system can be efficiently combined with a target site in a mammalian cell; and meanwhile, the Type I-F CRISPR/ Cas system is subjected to mammalian expression system optimization, so that a transcription activation factor or a transcription inhibition factor can be efficiently recruited to the target site in the mammalian cell, and a transcription activation system anda transcription inhibition system are successfully constructed. The application of the Type I-F CRISPR/ Cas system in the aspect of gene regulation and control of the mammalian cell becomes possible,and a necessary tool is provided for gene transcription regulation and control based on the Type I-F CRISPR/ Cas system.
本发明公开了一种基于Type I-F CRISPR/Cas的基因表达调控方法和基因表达调控系统。本发明首次研究显示隶属于Type I-F CRISPR/Cas系统的Cascade复合物，如PaeCascade复</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Gene expression regulation and control method and system based on Type I-F CRISPR/ Cas |
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