Gene expression regulation and control method and system based on Type I-F CRISPR/ Cas

The invention discloses a gene expression regulation and control method and system based on Type I-F CRISPR/ Cas. The first study shows that a Cascade compound, such as a PaeCascade compound, belonging to a Type I-F CRISPR/ Cas system can be efficiently combined with a target site in a mammalian cell; and meanwhile, the Type I-F CRISPR/ Cas system is subjected to mammalian expression system optimization, so that a transcription activation factor or a transcription inhibition factor can be efficiently recruited to the target site in the mammalian cell, and a transcription activation system anda transcription inhibition system are successfully constructed. The application of the Type I-F CRISPR/ Cas system in the aspect of gene regulation and control of the mammalian cell becomes possible,and a necessary tool is provided for gene transcription regulation and control based on the Type I-F CRISPR/ Cas system. 本发明公开了一种基于Type I-F CRISPR/Cas的基因表达调控方法和基因表达调控系统。本发明首次研究显示隶属于Type I-F CRISPR/Cas系统的Cascade复合物，如PaeCascade复