Genetic transformation method for insect-resistant gene transgenic Narrow-crown Populus Nigra No.11

The invention discloses a genetic transformation method for insect-resistant gene transgenic Narrow-crown Populus Nigra No.11, and belongs to the technical field of plant genetic engineering. The genetic transformation method comprises the processes of explant selection and pre-culture, agrobacterium infection, tissue culture seedling formation through induction culture and the like. A large number of high-quality trunk borer-resistant Narrow-crown Populus Nigra No.11 new strains can be cultivated in a short time by utilizing the genetic transformation method, the transformation time can be shortened, and the genetic transformation efficiency can be improved; and moreover, the transformation system is stable, is not influenced by time and seasons, and can be used for cultivating and producing excellent populus nigra strains with trunk borer resistance for northern China on a large scale. 本发明公开了一种转抗虫基因窄冠黑杨11号的遗传转化方法，属于植物基因工程技术领域。本发明的遗传转化方法包括：外植体选择及预培养、农杆菌侵染、诱导培养形成组培苗等过程，利用本发明的遗传转化方法可在短时间内培育出大量优质的抗蛀干害虫窄冠黑杨11号新品