Method for determining content of four alkaloids of uncaria rhynchophylla in Qingda granules

The invention provides a method for determining the content of four alkaloids of uncaria rhynchophylla in Qingda granules. The method comprises the following steps: preparing a test solution, preparing a reference solution and determining by adopting an ultra-high performance liquid chromatograph, wherein the preparation of the test solution comprises the following steps: taking Qingda granules under a loading amount difference item; grinding, precisely weighing, adding methanol, weighing, carrying out ultrasonic treatment, cooling, weighing again, supplementing the reduced weight with methanol, shaking up, filtering, precisely absorbing subsequent filtrate, passing through an alkaline alumina column, adding an elution solvent for elution, collecting effluent and eluent, drying by distillation, adding a redissolving solvent into residues, dissolving, and shaking up. The method provided by the invention solves the problem of full extraction of uncaria related components from Qingda granules, reduces the damage