Umbilical cord mesenchymal stem cell serum-free medium and preparation method thereof

The invention provides a serum-free medium for umbilical cord mesenchymal stem cells. The serum-free medium comprises vitamin H, transferrin, serum albumin, platelet-derived growth factors, fibroblastgrowth factors, epidermal growth factors, transforming growth factors, nanoparticle cholesterol, apigenin and methyl jasmonate. The adherence performance and the proliferation rate of the mesenchymalstem cells can be remarkably improved, the teratogenesis rate of the cells is effectively reduced, and the problems that in the prior art, a conventional serum or protein culture medium is low in survival rate and insufficient in algebraic quality are further solved. 本发明提供一种脐带间充质干细胞无血清培养基，包括维生素H，转铁蛋白，血清白蛋白，血小板衍生生长因子，成纤维细胞生长因子，表皮生长因子，转化生长因子，纳米粒子胆固醇，芹菜素以及茉莉酮酸甲酯；可显著提高间充质干细胞的贴壁性能和增殖速率，并有效降低细胞致畸率，还解决了现有技术存在的常规血清或蛋白培养基存在存活率低且代数品质不够的问题。