Cardiac troponin I recombinant antigen and preparation method of monoclonal antibody of cardiac troponin I recombinant antigen

The invention discloses a cardiac troponin I recombinant antigen. The cardiac troponin I recombinant antigen is similar to a natural cTnI antigen determinant and conformation, has very strong immunogenicity and immunoreactivity, and has higher secretory expression quantity and biological activity compared with full-length or segmented recombinant cTnI and other sequence splicing modes. The invention discloses a preparation method of a cardiac troponin I monoclonal antibody. The cell strain is prepared by combining the cardiac troponin I recombinant antigen with an immunological method and a hybridoma technology, so that a large number of specific monoclonal antibodies can be quickly obtained, and the obtained cardiac troponin I monoclonal antibodies are stable, relatively high in secretionamount and relatively high in biological activity, specificity and sensitivity, and can be used for detecting clinical serum. 本发明公开了一种心肌肌钙蛋白I重组抗原，该心肌肌钙蛋白I重组抗原与天然cTnI抗原决定簇以及构象相似，具有很强的免疫原性和免疫反应性，与全长或者分段重组cTnI以及其他序列拼接方式相比，具有更高的