Method for detecting antiviral performance of material through virus fluorescent protein expression

The invention discloses a simple and convenient-to-observe detection method of an antiviral material. Gene of green fluorescent protein (GFP) or other fluorescent proteins is cloned in virus to prepare recombinant virus. After the material is incubated with the virus with the fluorescent gene, virus liquid is collected to infect cells, and the infection ability of the virus is identified by detecting the expression condition of GFP or other fluorescent proteins of the cells. If the material can inactivate the virus and the virus cannot infect the cells, no GFP or other fluorescent proteins areexpressed in the cells, and the cells do not show fluorescence; If the material cannot inactivate the virus and the virus can infect the cells, GFP or other fluorescent proteins are expressed in thecells, and the cells show fluorescence. The method can simply and conveniently observe the antiviral performance of the material. 本发明公开了一种简单、方便观察的抗病毒材料的检测方法。将绿色荧光蛋白(GFP)或者其他荧光蛋白的基因克隆在病毒中制备重组病毒。材料与具有荧光基因的病毒孵育后，收集病毒液感染细胞，通过检测细胞G