Low-temperature culture medium for pichia pastoris

The invention discloses a low-temperature culture medium for pichia pastoris. The culture medium is favorable for realizing high-density fermentation of the pichia pastoris under the condition of lowtemperature (18-22 DEG C) while increasing the expression quantity of unit thalli. Various proteases in fermentation broth can be inhibited, so that the stability of the product structure can be maintained, and the generation of related impurities of the product can be reduced; When the method is applied to industrial preparation, the expression yield of exogenous proteins can be remarkably increased, and the downstream purification treatment difficulty is reduced. The preparation cost and the processing difficulty of products such as genetically engineered drugs, enzyme preparations and the like taking the pichia pastoris as the host are reduced. 本发明公开了一种巴斯德毕赤酵母低温型培养基，该培养基有助于在低温(18～22℃)条件下，在提高单位菌体表达量的同时，实现毕赤酵母菌的高密度发酵；还能够对发酵液中的各类蛋白酶起到抑制作用，有利于维系产物结构的稳定性，减少产品有关杂质的生成；在应用于工业化制备时，能够显著提高外源蛋白质的表达产量，并降低下游纯化处理难度。降低以毕赤酵母为宿主