Specific primer for quantitatively detecting transgenic soybean ZH10-6 and method

The invention discloses a specific primer for quantitatively detecting transgenic soybean ZH10-6. A pair of specific primers and a DNA polymerase with strand displacement activity are adopted, a fluorescently labeled probe is added into a PCR reaction system to monitor the PCR reaction process in re...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	LIU SHUANG, LAN QINGKUO, ZHAO XIN, WANG YIHENG, WEI WANCONG, WANG YONG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention discloses a specific primer for quantitatively detecting transgenic soybean ZH10-6. A pair of specific primers and a DNA polymerase with strand displacement activity are adopted, a fluorescently labeled probe is added into a PCR reaction system to monitor the PCR reaction process in real time, and nucleic acid is amplified at about 60 DEG C. A standard curve is made by diluting a standard substance, the percentage content of a transgenic herbicide-resistant soybean ZH10-6 transformant sequence is corrected by utilizing an internal reference, and an unknown sample is subjected torelative quantitative detection. For the result identification, whether amplification occurs or not is judged by judging whether an amplification curve is peaked or not and judging the cycle number ofpeaking, and the percentage content of an unknown sample is relatively quantified by utilizing the standard curve made by the standard substance. The detection method disclosed by the invention has the advantages of high spec