Organ-like lung cancer PDXO model, EGFR engineering modification and application of organ-like lung cancer PDXO model in pharmacodynamic research of tumor medicines

The invention relates to the technical field of biological medicines, in particular to an organ-like lung cancer PDXO model, EGFR engineering modification and an application of the organ-like lung cancer PDXO model in pharmacodynamic research of tumor medicines. The invention discloses a method for establishing an organ-like lung cancer PDXO model and a method for carrying out EGFR modification onthe PDXO model, and provides a method for rapidly converting a PDX model into an organ-like model and generating an engineering lung cancer organ-like model of an EGFR mutant by using gene modification. The constructed organ-like models can be cultured or stored for a long time, serve as lung cancer models, well simulate the growth characteristics and the microenvironment of three-dimensional tumors and are used for preclinical medicine development and research. 本发明涉及生物医药技术领域，具体涉及一种类器官肺癌PDXO模型以及EGFR工程化修饰及其在肿瘤药物药效学研究中的应用；本发明通过公开一种类器官肺癌PDXO模型的建立方法，以及对于此PDXO模型进行EGFR修饰的方法，提供了一种快速从PDX模型转化为类器官模型，并使用基因修饰产生EGFR突变体的工程化肺癌类器官模型