Method for calculating cell unit average toxin yield of AIV in MDCK-sus cells

The invention belongs to the technical field of microbial engineering, and particularly relates to a method for calculating cell unit average toxin yield of AIV in MDCK-sus cells, which comprises thefollowing steps: 1: acquiring density CDI and MOI of living cells when influenza viruses are infected; 2, obtaining the volume Vi of the residual influenza virus liquid after the ith sampling, and obtaining the hemagglutination titer value alpha HAi of the influenza virus at the moment i; 3, acquiring the chicken erythrocyte density RBC when the influenza virus hemagglutination titer is detected;4, according to the data obtained in the steps 1-3, obtaining the VPC of the cell unit, wherein the calculation formula of the VPC is as shown in the following formula 1; according to the method, based on the residual volume, titer, chicken erythrocyte density and MOI infection complex number after sampling in a designed time period, the average cell toxicity production amount in a certain time period is obtained, and the