Kit for detecting haemophilus influenzae through loop-mediated isothermal amplification and using method

The invention discloses a kit for detecting haemophilus influenzae through loop-mediated isothermal amplification. The kit comprises an upstream external primer F3, wherein the sequence is shown as SEQ ID NO: 1; a downstream external primer B3, wherein the sequence of the downstream external primer B3 is as shown in SEQ ID NO: 2; an upstream internal primer FIP, wherein the sequence of the upstream internal primer FIP is as shown in SEQ ID NO: 3; a downstream internal primer BIP, wherein the sequence of the downstream internal primer BIP is as shown in SEQ ID NO: 4, and a loop primer LF, wherein the sequence of the loop primer LF is as shown in SEQ ID NO: 5. The kit disclosed by the invention has the characteristics of strong specificity, high sensitivity and the like, is simple to operate, low in cost and short in consumed time when used for detecting haemophilus influenzae, and is suitable for popularization and application in underdeveloped area medical units. 本发明公开了一种环介导等温扩增检测流感嗜血杆菌的试剂盒，包括上游外部引物F3，其序列如SEQ