Monoclonal antibody prepared from African swine fever virus truncated protein p54 and application of monoclonal antibody

The invention belongs to the technical field of clinical immunology, and discloses a monoclonal antibody prepared from African swine fever virus truncated protein p54 and application of the monoclonalantibody. The monoclonal antibody clone antibody 4-4C is finally obtained by immunizing the effectively truncated African swine fever virus protein p54 and systematically screening hybridoma. The obtained monoclonal antibody can react with the African swine fever totivirus to meet the basic requirements of the monoclonal antibody; in addition, the monoclonal antibody is good in specificity and has no cross reaction with other proteins of the African swine fever virus; in addition, after the monoclonal antibody is detected by Elisa, the titer of the monoclonal antibody reaches up to 1600000; finally, the monoclonal antibody has a blocking effect, the established blocking Elisa detection method has a detection effect equivalent to that of a Spanish detection kit recommended by OIE, the detection time is shortened,