Method for improving in-vitro fertilization effect of unfrozen pig sperms

The invention discloses a method for improving the in-vitro fertilization effect of unfrozen pig sperms. The method comprises the following operation steps: (1) taking out frozen sperms, and unfreezing the frozen sperms; (2) cleaning the unfrozen seminal fluid with fertilization operating fluid, cen...

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Bibliographische Detailangaben
Hauptverfasser:	SUN HUA, LI ZHAOHUA, ZHANG ZHIBIN, LIU XIANGGUANG, WANG JUNHENG, JIAN XINRUI, ZHANG XIN, QIAN FURONG, LIU HAIXING, LI YANCHUN, GAO YI, WANG JUN, CAO YANG, GAO JIANHONG, ZHANG LICHUN, ZHAORIGETU, JIN QINGGUO, MENG QINGZHU, ZHU YONGKAI, DING DE, LU YONGHUI, YU YONGSHENG, MA TENGFEI, LI CHUANG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a method for improving the in-vitro fertilization effect of unfrozen pig sperms. The method comprises the following operation steps: (1) taking out frozen sperms, and unfreezing the frozen sperms; (2) cleaning the unfrozen seminal fluid with fertilization operating fluid, centrifuging twice, cleaning centrifugal precipitate with mTBM energetic fluid, centrifuging once, resuspending, and culturing to obtain energetic seminal fluid; (3) picking out a cumulus-oocyte complex from the ovary from which the follicular fluid is extracted under a microscope, culturing, taking out oocytes, and removing the cumulus; and (4) dropwise adding the obtained seminal fluid into each small fertilization cell, putting the small fertilization cells into an incubator for culture, and then washing off sperms attached to the oocytes to finish in-vitro fertilization culture. According to the invention, caffeic acid phenethyl ester and polyvinylpyrrolidone with corresponding concentrations are jointly added int