Normal-temperature mechanical perfusate for improving DCD liver supply of rats

The invention belongs to the field of medical treatment, and particularly relates to normal-temperature mechanical perfusate for improving DCD liver supply of rats. The perfusate is prepared from L-ascorbic acid, glucose, heparin, insulin, L-glutamine, antibiotics and a William's medium E culture medium. A normal-temperature mechanical perfusion platform is used, the perfusate is used for carryingout interventional treatment on rat DCD donor liver in vitro and analyzing and evaluating tissue morphology, related enzymes and metabolites in the perfusate, related cytokine secretion conditions and the like in the interventional treatment process, and results show that the perfusate has a remarkable improvement effect on rat DCD donor liver. The feasibility of the normal-temperature mechanicalperfusate rich in L-ascorbic acid and other components for improving the DCD edge donor liver is verified, and a reference and theoretical basis is provided for normal-temperature mechanical perfusion intervention treatment o