Bovine-derived anti-staphylococcus aureus single-chain antibody and preparation and application thereof
The invention discloses a bovine-derived anti-staphylococcus aureus single-chain antibody and preparation and application thereof, and relates to the field of single-chain antibody genetic engineering. The single-chain antibody at least comprises a bovine antibody light chain variable region (VL), a...
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| Format: | Patent |
| Sprache: | chi ; eng |
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| Zusammenfassung: | The invention discloses a bovine-derived anti-staphylococcus aureus single-chain antibody and preparation and application thereof, and relates to the field of single-chain antibody genetic engineering. The single-chain antibody at least comprises a bovine antibody light chain variable region (VL), a bovine antibody heavy chain variable region (VH) and an intermediate linker peptide (Linker), and the connection sequence is VL-Linker-VH. The preparation method comprises the following steps of: obtaining a bovine-derived single-chain antibody gene, constructing a bovine-derived single-chain antibody primary library, enriching and elutriating, and constructing a single-chain antibody prokaryotic expression plasmid pGEX-4T-1-scFv to obtain a single-chain antibody prokaryotic expression protein.The composition can be used for treating bovine mastitis caused by staphylococcus aureus. The single-chain antibody disclosed by the invention can be specifically combined with staphylococcus aureusLukE protein to inhibit th |
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