2019-nCoV nucleic acid isothermal amplification primer based on molecular beacon, molecular beacon, test kit and detection method

The invention discloses a 2019-nCoV nucleic acid isothermal amplification primer based on a molecular beacon, the molecular beacon, a test kit and a detection method. The 2019-nCoV virus primer comprises an ORF1ab gene primer sequence and an N gene primer sequence of a 2019-nCoV virus; the molecular beacon comprises an ORF1ab gene molecular beacon probe and an N gene molecular beacon probe; the test kit comprises the 2019-nCoV virus primer, a molecular beacon, an NASBA amplification buffer solution and an NASBA enzyme mixed solution, the detection method using the test kit comprises the following steps: firstly, preparing an NASBA amplification buffer solution, preparing an NASBA enzyme mixed solution, preparing a primer mixed solution, and preparing an RNA sample; mixing, reacting and incubating all the solutions, and finally completing detection through a fluorescence detector. The invention shows high-specificity and high-sensitivity detection performance, and a large amount of experiment time is saved. 本发