Porcine pathogenicity escherichia coli bivalent LTB toxin as well as preparation process and application thereof

The invention relates to the technical field of biological vaccine, in particular to porcine pathogenicity escherichia coli bivalent LTB toxin as well as a preparation process and an application thereof; the nucleotide sequence of the porcine pathogenicity escherichia coli bivalent LTB toxin gene is shown as SEQ ID No: 1. The invention also discloses a specific antibody which is combined with a swine fever spleen drenching vaccine to prepare a bivalent vaccine, so that the specific antibody level of the swine fever can be obviously enhanced, and the diarrhea rate of suckling piglets can be reduced; effects of treating one aspect in a targeted manner and preventing two aspects is achieved, the toxin is more efficient, more economical and safer, the application of antibiotics in the aspect of treatment is effectively reduced, and a series of problems caused by excessive use of the antibiotics are reduced. 本发明涉及生物疫苗技术领域，尤其是一种猪致病性大肠杆菌双价LTB毒素及制备工艺和应用，所述猪致病性大肠杆菌双价LTB毒素基因核酸序列如SEQ ID No：1所示。本发明还制备了其特异性的抗体，并与猪瘟脾淋苗联合制备