High-hydrophilicity nucleic acid aptamer functional extraction material and application thereof

The invention discloses a high-hydrophilicity nucleic acid aptamer functional extraction material and application thereof, and belongs to the technical field of analytical chemistry. The preparation method comprises the following steps: using N, N-methylene bisacrylamide as a high-hydrophilicity cross-linking agent; taking 2-acrylamide-2-methylpropanesulfonic acid as a high-hydrophilicity organicpolymerization monomer and N, N-dimethylformamide, PEG 10000 and water as pore-foaming agents, immobilizing a PAEs nucleic acid aptamer through a mercapto-ene click chemical reaction by adopting a one-pot method, and carrying out free radical thermal polymerization to form the extraction material. The extraction material can be well applied to detection and analysis of PAEs in drinking water, anda new way is provided for separation and specific recognition of hydrophobic substances. 本发明公开了一种高亲水性核酸适配体功能萃取材料及其应用，属于分析化学技术领域。其是以N,N-亚甲基双丙烯酰胺作为高亲水交联剂，2-丙烯酰胺-2-甲基丙磺酸作为高亲水有机聚合单体，N,N-二甲基甲酰胺、PEG 10000和水作为致孔剂，采用一锅法，通过"巯基-烯"点击化学反应