Method and recombinant promoters for efficiently expressing high-temperature-resistant alpha-amylase in bacillus subtilis, and application

The invention relates to the field of gene engineering, in particular to a method and recombinant promoters for efficiently expressing high-temperature-resistant alpha-amylase in bacillus subtilis, and application. The invention provides a series of combined promoters and signal peptides capable of efficiently expressing the high-temperature-resistant alpha-amylase. Compared with a control promoter and signal peptide combination, the relative enzyme activity of the high-temperature-resistant alpha-amylase of the optimized promoter and signal peptide combinations is improved to a certain extent, the enzyme activity of the high-temperature-resistant alpha-amylase of the hybrid promoter and different signal peptide combinations is greatly improved, and a foundation is laid for industrial production of the the high-temperature-resistant alpha-amylase. 本发明涉及基因工程领域，具体涉及在枯草芽孢杆菌中高效表达耐高温α-淀粉酶的方法、重组启动子以及应用。本发明提供了一系列能高效表达耐高温α-淀粉酶的组合启动子和信号肽，相对于对照启动子和信号肽组合，所有优化后的启动子与信号肽组合的耐高温α-淀粉酶相对酶活均有一定的提高，杂合启动子与不同信号肽组合的高温α-淀粉酶酶活力提高幅度更大