Method for constructing MDBK cell line with MyoD1 gene knocked out by using CRISPRCAS9 technology

Method for constructing MDBK cell line with MyoD1 gene knocked out by using CRISPRCAS9 technology

The invention discloses a method for constructing an MDBK cell line with MyoD1 gene knocked out by using a CRISPRCAS9 technology. According to the method, sgRNA virus liquid carrying the MyoD1 gene isused for infecting an MDBK cell to knock out the MyoD1 gene, and the MDBK cell with the MyoD1 gene k...

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Bibliographische Detailangaben
Hauptverfasser: TAN XIAOSHAN, MAO SHIMING, LI JUN, LONG QINGMENG, CHEN GUANGHOU, LI MINGYONG, ZHANG XIAOYU, SHEN JINRONG, ZHOU DI, XIE YAN, LI PING, ZHAO ZHONGHAI, LI JIANWEI, LIU XIAO, XIE LINGLING
Format: Patent
Sprache:chi ; eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Zusammenfassung:The invention discloses a method for constructing an MDBK cell line with MyoD1 gene knocked out by using a CRISPRCAS9 technology. According to the method, sgRNA virus liquid carrying the MyoD1 gene isused for infecting an MDBK cell to knock out the MyoD1 gene, and the MDBK cell with the MyoD1 gene knocked out can be used for downstream research of functions of the MyoD1 gene and related regulation mechanisms or pathways. 本发明公开了一种CRISPRCAS9技术构建MyoD1基因敲除的MDBK细胞系的方法，该方法利用携带MyoD1基因的sgRNA病毒液感染MDBK细胞敲除MyoD1基因，MyoD1基因敲除的MDBK细胞可于下游研究MyoD1基因的功能及与其相关的调控机制或通路。