Method for separating and detecting isomaltulose in food

The invention discloses a method for separating and detecting isomaltulose in food, which comprises the following steps: dispersing a sample in water, removing protein, carrying out microporous filtration, separating the filtrate by using an amide bonded chromatographic column, and taking acetonitrile/deionized water as the mobile phase for separation. According to examples of some separation methods, an amide bonded chromatographic column which is relatively weak in sugar separation capacity but better in stability is adopted, and a specific elution procedure is matched, so that sucrose and isomaltulose in a sample, such as food, can be completely separated; and the detection line of the isomaltulose is as low as 50 [mu] g/mL. According to examples of some detection methods, the working curve linearity is good within 50-1000 ug/mL, the correlation coefficient r2 is equal to 0.9993, and the recovery rate ranges from 89.3% to 98.3%. And the precision (n is equal to 6) is 2.18%-2.58%. Meanwhile, the method is s