Method for separating and extracting primary cancer cells of cutaneous squamous cell carcinoma

The invention provides a method for separating and extracting primary cancer cells of cutaneous squamous cell carcinoma. The obtained primary cancer cells of cutaneous squamous cell carcinoma are highin success rate of separation and extraction, low in degree of culture pollution, large in number and high in quality. A large number of cutaneous squamous cell carcinoma cells can be obtained by subculture. Under a microscope, the primary cells of cutaneous squamous cell carcinoma show typical epithelioid cells and fibroblast-like cells. Compared with conventionally cultured cutaneous squamous cell carcinoma cells, the obtained cutaneous squamous cell carcinoma cells have higher biological activity and higher growth rate. 本发明提供了一种皮肤鳞癌原代肿瘤细胞的分离提取方法，获得的皮肤鳞癌原代细胞不仅分离提取的成功率高，培养污染的发生程度低，而且获得原代细胞数量多，质量高。经传代培养可获得大量的皮肤鳞癌细胞。显微镜下皮肤鳞癌原代细胞有典型的上皮样细胞和成纤维样细胞。相比于常规培养的皮肤鳞癌细胞，本发明所获得皮肤鳞癌细胞生物活性更高，生长速率更快。