Group I fowl adenovirus type 4 SD-F strain, inactivated vaccine and preparation method

The invention provides a group I fowl adenovirus type 4 SD-F strain, an inactivated vaccine and a preparation method, and belongs to the field of bioengineering. The preservation number of the group Ifowl adenovirus type 4 SD-F strain is CCTCC NO: V201964. The preparation method of the fowl adenovirus inactivated vaccine comprises the following steps: inactivating a group I avian adenovirus type4 SD-F strain virus liquid, then mixing inactivated liquid with tween-80, and performing uniform stirring and mixing to obtain a water phase; mixing white oil with span-80, and performing uniform stirring and sterilizing to obtain an oil phase; and mixing the water phase and the oil phase according to a volume ratio of 1:(2-4), and performing emulsifying to obtain the inactivated vaccine. The SD-Fstrain disclosed by the invention can adapt to DF-1 cells without tumorigenicity, and is simple in preparation process, low in cost, free of tumorigenicity risk and relatively high in safety; and theinactivated vaccine is high