NIR-II imaging probe and preparation method and application thereof

The invention discloses a NIR-II imaging probe and a preparation method and application thereof, and belongs to the technical field of in vivo bioluminescence imaging of cells. The invention comprisesthe following steps: S1, incubating bone marrow mesenchymal stem cells (BMSCs) of fluorescent probe CP@mSiO2(subscript)-PEG nanoparticles; S2, injecting fluorescent material-labeled BMSCs into HepG2tumor-bearing nude mice through tail vein injection and local subcutaneous injection; and S3, carrying out near-infrared-II (NIR-II) window imaging on the tumor, and then performing imaging-guided tumor resection. In the study, NIR-II is a new strategy for tumor imaging in vivo and image-guided tumor resection surgery, the NIR-II in vivo bioluminescence imaging technology is used to induce the fluorescent material-labeled BMSCs to enrich in the tumor site, so that the outline of the tumor can be seen clearly, thus improving the accuracy of surgical resection and reducing the tumor recurrence rate and the mortality rate