Method for simultaneously detecting contents of tin and lead in different forms in tobacco
The invention provides a method for simultaneously detecting the contents of tin and lead in different forms in tobacco. The method comprises the following steps: extracting tobacco to be determined by using a sample treatment solution, separating to obtain a supernatant, concentrating, and fixing t...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention provides a method for simultaneously detecting the contents of tin and lead in different forms in tobacco. The method comprises the following steps: extracting tobacco to be determined by using a sample treatment solution, separating to obtain a supernatant, concentrating, and fixing the volume by using a mixed solution of a mobile phase A and a mobile phase B to obtain a to-be-detected sample solution, wherein the sample treatment solution is a mixed solution of a nitric acid aqueous solution and methanol, the mobile phase A is a nitric acid aqueous solution containing sodium hexanesulfonate, and the mobile phase B is methanol; meanwhile, performing a contrast test to obtain a blank to-be-detected sample solution; analyzing a series of standard solutions containing tin and lead in different forms by using an HPLC-ICP-MS, and making a standard curve; and analyzing the to-be-detected sample solution and the blank to-be-detected sample solution by using the HPLC-ICP-MS to obtain chromatographic pe |
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