Primer group and probe group for DuHCV and DuMV dual real-time fluorescent quantitative PCR detection

The invention relates to a primer group and a probe group for DuHCV and DuMV dual real-time fluorescent quantitative PCR detection. The sequences of the primer group and the probe group are shown as SEQ. ID. No. 1 to SEQ. ID. No. 6. A dual TaqMan real-time fluorescent quantitative PCR detection method established by utilizing the primer group and the probe group can be used for simultaneously detecting and accurately quantifying DuHCV and DuMV popular in duck flocks, the operation procedure is simplified, the cost is saved, detection is rapid and efficient, the specificity is high, the sensitivity is also high, and the lowest detection limit of DuHCV is 72.1 copies/mu L; and the lowest detection limit of DuMV is 60.8 copies/mu L. 本发明涉及一种用于DuHCV和DuMV双重实时荧光定量PCR检测的引物组和探针组，所述引物组和探针组的序列如SEQ.ID.No.1-SEQ.ID.No.6所示，本发明利用所述引物组和探针组建立的双重TaqMan实时荧光定量PCR检测方法，能够同时对鸭群中流行的DuHCV和DuMV的进行检测和精确定量，简化操作程序、节约成本、检测快速、高效、特异性强且灵敏度高，DuHCV的最低检测限为72.1拷贝/μL；DuMV的最低检测限为60.8拷贝/μL。