Multiplex PCR primer combination, reagent and multiplex PCR interrupt control system for whole exome second-generation sequencing of BRAF gene

The invention provides a multiplex PCR primer combination, reagent and multiplex PCR interrupt control system for whole exome second-generation sequencing of BRAF gene. The multiplex PCR primer combination for whole exome second-generation sequencing of the BRAF gene comprises primers shown in SEQ ID NO: 01-SEQ ID NO: 36. The multiplex PCR primer combination, reagent and multiplex PCR interrupt control system for whole exome second-generation sequencing of the BRAF gene solve the following existing technical problems: 1, point mutations of the BRAF gene are mainly detected in the existing technology, and the detection range is limited; 2, only known sites can be detected by real-time fluorescence quantitative PCR detection and gene chips; 3, test length of Sanger sequencing is shorter than800 bp; 4, low frequency mutations cannot be detected; and 5, operation is cumbersome and expensive when multiple sites are detected. 本发明提供一种用于BRAF基因全外显子二代测序的多重PCR引物组合物、试剂及多重PCR-打断控制系统。所述用于BRAF基因全外显子二代测序的多重PCR引物组合物包括SEQ ID N