Plant linkage expression vector for inhibiting OsHIS1 gene expression as well as construction method and application of plant linkage expression vector

The invention discloses a plant linkage expression vector for inhibiting OsHIS1 gene expression. The plant linkage expression vector comprises an element for inhibiting OsHIS1 gene expression, a fertility restoring gene element, a pollen lethal gene element and a fluorescence labeling gene element. The invention further discloses a construction method of the plant linkage expression vector. Afterthe sterile line is transformed by the obtained plant linkage expression vector, a reproductive line containing transgenic ingredients, which is missed in color selection, can be killed by beta triketone herbicides, the transgenic effect is accurate, and the impurity removal efficiency is high. The invention further discloses application of the plant linkage expression vector in third-generation hybrid rice seed production or mechanical mixed sowing and mixed harvesting seed production. Hybrid seed production and obtained hybrid seed transgenosis pollution risks caused by color sorting missingdetection of sterile line